high prevalence of icaabcd genes responsible for biofilm formation in clinical isolates of staphylococcus aureus from hospitalized children

نویسندگان

abdolmajid ghasemian department of bacteriology, tarbiat modares university, tehran, ir iranسازمان اصلی تایید شده: دانشگاه تربیت مدرس (tarbiat modares university)

shahin najar-peerayeh department of bacteriology, tarbiat modares university, tehran, ir iran; department of bacteriology, tarbiat modares university, tehran, ir iran. tel: +98-92182883870سازمان اصلی تایید شده: دانشگاه تربیت مدرس (tarbiat modares university)

bita bakhshi department of bacteriology, tarbiat modares university, tehran, ir iranسازمان اصلی تایید شده: دانشگاه تربیت مدرس (tarbiat modares university)

mohsen mirzaee department of laboratory sciences, borujerd branch, islamic azad university, borujerd, ir iranسازمان اصلی تایید شده: دانشگاه آزاد اسلامی بروجرد (islamic azad university of borujerd)

چکیده

conclusions prevalence of icaadbc genes was relatively high among children and also all the four ica genes were detected among mrsa strains. results five isolates (22.7%) were methicillin resistant (mrsa) and meca gene was detected among them. all the mrsa isolates harbored sccmec type iii. prevalence of icaa, icab, icac and icad in the isolates were 16 (73%), 14 (63.6%), 16 (73%) and 16 (73%), respectively. moreover, all the mrsa strains were icaadbc positive. background the icaabcd genes encode a polysaccharide intercellular adhesion (pia), which is a tight structure protecting staphylococcus aureus community against adverse environmental conditions. the ica dependent biofilm formation plays an important role in persistent infections in hospitalized patients. objectives the aim of this study was to detect icaadbc genes encoding pia among s. aureus isolates from children in loghman hospital of tehran. materials and methods we collected 22 clinical specimens from hospitalized pediatrics and identified the isolates. then, we detected meca gene among methicillin resistant s. aureus (mrsa), sccmec types and icaabcd gens by pcr assay and specific primers.

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عنوان ژورنال:
archives of pediatric infectious diseases

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